小分子滴蛋白，比例10:1，濃度蛋白0.1，小分子可以1mM。一般小分子滴大分子，小分子100uM的話，大分子就是10uM。小分子(100uL)滴定大分子(500uL)一次的體積，另外單獨(dú)需要1mL緩沖液加參比池和潤洗樣品池、針頭。盡量b證蛋白和小分子溶劑一致。

　　吸收熱量一般在多大值合適：吸收熱量沒有多大合適，太低了會(huì)沒有辦法模擬曲線。也沒有多大合適，因?yàn)椴煌姆磻?yīng)類型熱量差異很大。

　　滴定速度是否能快一點(diǎn)：一般滴一滴就要10秒左右，常規(guī)滴19滴。19滴z快也要3分多，而且中間不一定平穩(wěn)，不建議滴定快的。

　　ITC測(cè)試軟件：MicroCal iTC200 (GE Healthcare, USA)。

　　常規(guī)測(cè)試的條件以及儀器型號(hào)：ITC experiments were performed using a MicroCal iTC200 (GE Healthcare, USA). The reference power was set to 1.2 μcal/s, and the stirring speed was 500rpm. A series of 19 injections of 2 μL was performed at 180-s intervals at 25 °C. Parallel control experiments were performed. Data were analysed using MicroCal-enabled Origin software (OriginLab, USA).